Sequencing Facility
The Sequencing Center provides faculty and students with a centralized resource where multiple users have the opportunity to access automated nucleic acid sequencing and analysis instrumentation. Located within the Sequencing Facility is an Applied Biosystems 3730xl DNA Analyzer, whose 96-capillary array allows researchers to perform high-throughput, large-scale nucleic acid sequencing and analysis. An additional Applied Biosystems 3100 Genetic Analyzers, equipped with a 16-capillary array, offers further resources for users who are working on smaller-scale sequencing and fragment analysis applications. Also available within the facility is a Roche LightCycler 480 Real-Time PCR machine.
The following rate schedule applies to all trained, eligible users within the the College of Life Sciences and is current for the 2006/2007 Academic Year. Rates are adjusted each year depending on usage. Fees are used to help cover the cost of the service contracts on the instruments, which are partially subsidized by CBMG, the Biology department and the College of Life Sciences, and to help pay for materials such as polymer, buffer and capillary arrays.
| Instrument | Rate |
| 3730xl South DNA Analyzer | $35.00/run (96 wells) |
| 3100 West Genetic Analyzer | $12.50/run (16 wells) |
| Roche LightCycler 480 Real-Time PCR Machine | $7.00/hour (1 plate) |
The Sequencers and the Roche LightCycler 480 Real-Time PCR machine are available to trained, eligible users. Those who wish to reserve time on either instrument must contact the facility manager for training and sign up for run time using Oracle Calendar. New users who do not have an Oracle Calendar account will need to apply for a single account.
Access to the Sequencing Center, located in room 0107 of the Microbiology Building, is controlled with a card lock that grants access by reading the barcode of a University identification card. Anyone requiring entry into this area should contact the facility manager for access.
Users who have reserved a block of time on an instrument are responsible for reporting for their scheduled session. All users are encouraged to familiarize themselves with the complete list of policies governing the use of the facility.
Amy Beaven
Manager
Core Laboratory Facility
0107 Microbiology Building
University of Maryland
abeaven@umd.edu
301-405-7238 | 
